Scanning the system in Safe Mode is good practice, but some malware can survive even in Safe Mode. A BartPE Bootable CD works well for these stubborn Windows infectors. To create the BartPE Bootable CD, you'll need your licensed, original Windows installation CD. You'll also need about 500Mb of free disk space, which shouldn't be a problem with todays multi-gigabyte drives. And, of course, you'll need a CD/DVD burner.
BartPE supports Windows XP Home and Professional (SP1 or higher), and Windows Server (Web, Standard, and Enterprise editions).
For full instructions and the free download, check out: http://www.nu2.nu/pebuilder/
Note that if you like and use the BartPE Bootable CD, the author does accept donations. Look for the donation button near the bottom of the http://www.nu2.nu/pebuilder/ page.

Will it work with Windows 7??
Sadly, BartPE doesn’t support Vista or Windows 7.
what if you didnt get the original CD with the computer can you still continue to check for viruses?
Why don’t you try to boot any of live Linux/Unix CD without pebuilder? and
via samba execute any wins progs like antivirus portable?????